Table 1 The primers of UGT1A/DPYD variants genotypes
Mutation Type | PCR Primer (5′-3′) | Product length |
|---|---|---|
UGT1A1*6 [5] | Primer-F: ACGCCTCG TTGTACATCAGAG | 217 bp |
Primer-R: CCTTGTT GTGCAGTAAGTGG | ||
UGT1A1*27 | Primer-F: ACTTACTGCACAACAAGGAGCT | 484 bp |
Primer-R: CACACCTGGGATAGTGGATTTTG | ||
UGT1A1*28 [5] | Primer-F: AGCCAGTTCAACTGTTGTTGC | 208 bp |
Primer-R: TTTGCT CCTGCCAGAGGTTC | ||
UGT1A7*2/*3/*4 [28] | Primer-F: TTTGCCGATGCTCGCTGGACG | 415 bp |
Primer-R: GCTATTTCTAAGACATTTTTGAAAAAATAGGG | ||
UGT1A9*22 [35] | Primer-F: ACTTAACATTGCAGCACAGG | 556 bp |
Primer-R: ATGGGCAAAAGCCTTGAACT | ||
DPYD*5 | Primer-F: ATTCAGTTCACTGCTCACTGAC | 370 bp |
Primer-R: GAGAAAGTTTTGGTGAGGGCA | ||
DPYD c.1896 T > C, | Primer-F: TGGACAAAGCTCCTTTCTGAATA | 231 bp |
DPYD*2A [36] | Primer-R: CAGCAAAGCAACTGGCAGAT |