Table 1 List of antibodies
Antigen | Fluor | Clone | Isotype | Supplier | Catalogue # | Panel | Dilution |
|---|---|---|---|---|---|---|---|
BDCA-1 (CD1c) | PE | AD5-8E7 | ms IgG1 | Miltenyi | 130–090-508 | 1 | 1/10 |
BDCA-2 (CD303) | FITC | AC144 | ms IgG1 | Miltenyi | 130–090-510 | 1 | 1/10 |
BDCA-3 (CD141) | APC | AD5-14H12 | ms IgG1 | Miltenyi | 130–090-907 | 1 | 1/10 |
HLA-DR | V500 | G46.6 | ms IgG2a | BD Biosciences | 561,224 | 1 | 1/100 |
CD3 | PerCP-Cy5.5 | SK7 | ms IgG1 | Biolegend | 344,808 | 1 | 1/100 |
CD14 | PerCP-Cy5.5 | HCD14 | ms IgG1 | Biolegend | 325,622 | 1 | 1/100 |
CD16 | PerCP-Cy5.5 | 3G8 | ms IgG1 | Biolegend | 302,028 | 1 | 1/100 |
CD19 | PerCP-Cy5.5 | HIB19 | ms IgG1 | Biolegend | 2,072,925 | 1 | 1/100 |
CD56 | PerCP-Cy5.5 | HCD56 | ms IgG1 | Biolegend | 318,322 | 1 | 1/100 |
CD40 | APC-H7 | 5C3 | ms IgG1 | BD Biosciences | 561,211 | 1 | 1/10 |
CD4 | AF488 | RPA-T4 | ms IgG1 | BD Biosciences | 557,695 | 2 | 1/20 |
CD3 | PE | SK7 | ms IgG1 | BD Biosciences | 347,347 | 2 | 1/50 |
CD8 | PECy7 | RPA-T8 | ms IgG1 | BD Biosciences | 555,368 | 2 | 1/50 |
CD4 | APC-H7 | RPA-T4 | ms IgG1 | BD Biosciences | 560,158 | 3 | 1/40 |
Foxp3 | PE | PCH101 | rt IgG2a | eBioscience | 12–4776-42 | 3 | 1/20 |
CD25 | APC | M-A251 | ms IgG1 | BD Biosciences | 555,434 | 3 | 1/5 |
CD127 | PECy7 | eBioRDR5 | ms IgG1 | eBioscience | 25–1278-42 | 3 | 1/100 |
Ki67 | FITC | B56 | ms IgG1 | BD Biosciences | 556,026 | 3,4 | 1/10 |
ICOS | PerCP-Cy5.5 | C398.4A | ha IgG | Biolegend | 313,518 | 3,4 | 1/80 |
CD14 | V500 | M5E2 | ms IgG2a | BD Biosciences | 561,391 | 3,4 | 1/80 |
CD19 | V500 | HIB19 | ms IgG1 | BD Biosciences | 561,121 | 3,4 | 1/80 |
CD3 | V450 | UCHT1 | ms IgG1 | BD Biosciences | 560,365 | 3,4 | 1/40 |
CD8 | APC-H7 | SK1 | ms IgG1 | BD Biosciences | 560,179 | 4 | 1/40 |
CD38 | AF647 | HIT2 | ms IgG1 | Biolegend | 303,514 | 4 | 1/40 |
HLA-DR | PECy7 | L243 | ms IgG2a | BD Biosciences | 335,795 | 4 | 1/80 |
Bcl-2 | PE | Bcl-2/100 | ms IgG1 | BD Biosciences | 556,535 | 4 | 1/10 |