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Table 1 List of antibodies

From: Serial immunomonitoring of cancer patients receiving combined antagonistic anti-CD40 and chemotherapy reveals consistent and cyclical modulation of T cell and dendritic cell parameters

Antigen Fluor Clone Isotype Supplier Catalogue # Panel Dilution
BDCA-1 (CD1c) PE AD5-8E7 ms IgG1 Miltenyi 130–090-508 1 1/10
BDCA-2 (CD303) FITC AC144 ms IgG1 Miltenyi 130–090-510 1 1/10
BDCA-3 (CD141) APC AD5-14H12 ms IgG1 Miltenyi 130–090-907 1 1/10
HLA-DR V500 G46.6 ms IgG2a BD Biosciences 561,224 1 1/100
CD3 PerCP-Cy5.5 SK7 ms IgG1 Biolegend 344,808 1 1/100
CD14 PerCP-Cy5.5 HCD14 ms IgG1 Biolegend 325,622 1 1/100
CD16 PerCP-Cy5.5 3G8 ms IgG1 Biolegend 302,028 1 1/100
CD19 PerCP-Cy5.5 HIB19 ms IgG1 Biolegend 2,072,925 1 1/100
CD56 PerCP-Cy5.5 HCD56 ms IgG1 Biolegend 318,322 1 1/100
CD40 APC-H7 5C3 ms IgG1 BD Biosciences 561,211 1 1/10
CD4 AF488 RPA-T4 ms IgG1 BD Biosciences 557,695 2 1/20
CD3 PE SK7 ms IgG1 BD Biosciences 347,347 2 1/50
CD8 PECy7 RPA-T8 ms IgG1 BD Biosciences 555,368 2 1/50
CD4 APC-H7 RPA-T4 ms IgG1 BD Biosciences 560,158 3 1/40
Foxp3 PE PCH101 rt IgG2a eBioscience 12–4776-42 3 1/20
CD25 APC M-A251 ms IgG1 BD Biosciences 555,434 3 1/5
CD127 PECy7 eBioRDR5 ms IgG1 eBioscience 25–1278-42 3 1/100
Ki67 FITC B56 ms IgG1 BD Biosciences 556,026 3,4 1/10
ICOS PerCP-Cy5.5 C398.4A ha IgG Biolegend 313,518 3,4 1/80
CD14 V500 M5E2 ms IgG2a BD Biosciences 561,391 3,4 1/80
CD19 V500 HIB19 ms IgG1 BD Biosciences 561,121 3,4 1/80
CD3 V450 UCHT1 ms IgG1 BD Biosciences 560,365 3,4 1/40
CD8 APC-H7 SK1 ms IgG1 BD Biosciences 560,179 4 1/40
CD38 AF647 HIT2 ms IgG1 Biolegend 303,514 4 1/40
HLA-DR PECy7 L243 ms IgG2a BD Biosciences 335,795 4 1/80
Bcl-2 PE Bcl-2/100 ms IgG1 BD Biosciences 556,535 4 1/10
  1. List of monoclonal antibodies used for flow cytometric staining. Panels were used for dendritic cell staining of PBMC (panel 1), absolute cell counts of whole blood (panel 2), Treg staining of PBMC (panel 3), or CD8 T cell staining of PBMC (panel 4). Abbreviations: AF = AlexaFluor, ms = mouse, rt. = rat, ha = hamster