Table 1 Primers sequences
From: GABPA predicts prognosis and inhibits metastasis of hepatocellular carcinoma
Primer name | F:5′-3′ | R:5′-3’ | |
|---|---|---|---|
RT-PCR | GABPA | AAGAACGCCTTGGGATACCCT | GTGAGGTCTATATCGGTCATGCT |
E-cadherin | CGACCCAACCCAAGAATCTATC | AGGTGGTCACTTGGTCTTTATTC | |
β-actin | ATAGCACAGCCTGGATAGCAACGTAC | CACCTTCTACAATGAGCTGCGTGTG | |
shRNA | GABPA-1 | CCGGTGTTATCAGTAAGAAGTTCTAGCTTCAAGAGAGCTAGAACTTCTTACTGATAATTTTTTG | AATTCAAAAAATTATCAGTAAGAAGTTCTAGCTCTCTTGAAGCTAGAACTTCTTACTGATAACA |
GABPA-2 | CCGGTGATCTGGATCAATAACAACCTCTTCAAGAGAGAGGTTGTTATTGATCCAGATTTTTTTG | AATTCAAAAAAATCTGGATCAATAACAACCTCTCTCTTGAAGAGGTTGTTATTGATCCAGATCA | |
E-cadherin | GATCCGCACCAAAGTCACGCTGAATTTCAAGAGAATTCAGCGTGACTTTGGTGTTTTTTACGCGTG | AATTCACGCGTAAAAAACACCAAAGTCACGCTGAATTCTCTTGAAATTCAGCGTGACTTTGGTGCG | |
Pbobi-cmv | GABPA | GACTCTAGAGGATCCATGTACCCATACGACGTCCCAGACTACGCTACTAAAAGAGAAGCAGAGGAGC | AATTAATTCCTCGAGTTAATTATCCTTTTCCGTTTGCAGAGAAGC |
ChIP RT-PCR | E-Cadherin-P1 | CAGTTGCTATGATGAGCCAAGA | GGGAAGTCAGTGTTCTCCTTTG |
E-Cadherin-P2 | CTCTCATTGGCCTCAATCTCTC | GCCACTGACCAGCTCATTTA | |
E-Cadherin-P3 | ACCACGCCTGGCTAATTT | GATCACGAGGTCAGGAGATTG | |
E-Cadherin-P4 | CTCACTAACCCATGAAGCTCTAC | GCCGAGGCTGATCTCAAAT | |
E-Cadherin-P5 | CACCTGTACTCCCAGCTACTA | GGTCTCACTCTTTCACCCAAG |