Fig. 2

Impact of MG and a GLO1 inhibitor on colony formation of tumor cells. Western blot analysis with whole cell lysate demonstrates higher basal GLO1 expression in control treated (co) FaDu as compared to Cal27 cells and MG-induced up-regulation of GLO1 protein levels in both cell lines (a). Detection of β-Actin served as control for quantity and quality of protein lysates. b The cytotoxic effect of MG on FaDu (black line) and Cal27 cells (dashed line) was assessed by a colony-forming assay and the graph indicates the mean value ±SD of the survival fraction at the indicated MG concentration of three independent experiments. The impact of GLO1 inhibition (1 and 5 μM S-p-bromobenzylglutathione cyclopentyl diester) on the viability of FaDu and Cal27 cells was assessed by a colony-forming assay (b) and the graph in (c) displays the mean value ±SD of the survival fraction at the indicated concentration of the GLO1 inhibitor from three independent experiments