Skip to main content

Table 2 Primers used in second round PCR amplification

From: Is detection of intraperitoneal exfoliated tumor cells after surgical resection of rectal cancer a prognostic factor of survival?

#

Start (bp)

End (bp)

Template, fragment # from # from

“Forwards” primer (5′ - 3′)

“Reverse” primer (5′ - 3′)

Annealing temperature (°C)

1

16,569

25

1

aTGCATGGAGAGCTCCCGTGAGTGG

CCCCTTAAATAAGACATCACGAT

52

2

42

126

1

aATTAACCACTCACGGGAGCTCTC

AGGATGAGGCAGGAATCAAAGAC

55

4

131

181

1

aCACCCTATGTCGCAGTATCTGTC

CACACTTTAGTAAGTATGTTCGC

55

6

483

513

1

aGGGGTTAGCAGCGGTGTGTGTGTG

TCCCACTCCCATACTACTAATCT

55

7

530

633

1

aTACCCAGCACACACACACCGCTG

CAAACCTATTTGTTTATGGGGTGA

55

8

673

705

1

aTTAGAGGGTGAACTCACTGGAAC

GGTTTGGTCCTAGCCTTTCTATT

58

82

7031

7134

10

aACGACACGTACTACGTTGTAGCC

AATATGATAGTGAAATGGATTTT

52

84

7340

7416

10

aCTTTCTTCCCACAACACTTT CT C

TCTCAAATCATGAAAATTATTAAT

55

125

11,029

11,086

16

aTTAGGAGGGGGGTTGTTAGGGGGT

CATCCCTCTACTATTTTTTAACC

58

127

11,193

11,243

16

aACCAGCCAGAACGCCTGAACGCA

GGTGTTGTGAGTGTAAATTAGT G

55

128

11,283

11,311

16

aTGTGCCTGCGTTCAGGCGTTCTGG

TAATCATATTTTATATCTTCTTC

60

130

11,437

11,492

16

aTTGACCCAGCGATGGGGGCTTCGA

GAGCCAACAACTTAATATGACTA

55

176

15,201

15,257

22

aAGAATCGTGTGAGGGTGGGACTGT

AGTAATTACAAACTTACTATCCG

60

177

15,274

15,377

22

aAGTAGACAGTCCCACCCTCACAC

GGTGATTTTATCGGAATGGGAGG

60

178

15,394

15,448

22

aCTAGGAATCACCTCCCATTCCGA

TAATGTCATTAAGGAGAGAAGGAA

55

181

15,761

15,864

22

aACCTCCTCATTCTAACCTGAATC

CAGGCCCATTTGAGTATTTTGTTT

55

184

16,080

16,130

23

aCAAGTATTGACTCACCCATCAAC

ACAGGTGGTCAAGTATTTATGGTA

57

187

16,263

16,366

23

aAACTGCAACTCCAAAGCCACCCC

CCCTATCTGAGGGGGGTCATCCAT

58

  1. a=tail sequence (CCCGCCGCCCCCGCCCGGG)
  2. GC-Clamp = (6FAM-GCGCCCGCCGCGCCCCGCGCCCGTCC CGCCGCCCCCGCCCGGG)