The unique C- and N-terminal sequences of Metallothionein isoform 3 mediate growth inhibition and Vectorial active transport in MCF-7 cells

Table 2 TERs measured in various MCF-7 MT3 mutants

MCF-7 Cell Lines	Average TER Day 5 (Ω/cm²)	Average TER Day 6 (Ω/cm²)	Average TER Day 7 (Ω/cm²)	Average TER Day 5, 6 and 7 (Ω/cm²)
MT3	35.32 +/− 5.81	53.57 +/− 13.88	52.27 +/− 12.11	48.72
MT3ΔCT	41.99 +/− 9.26	53.08 +/− 9.57	47.67 +/− 5.16	47.58
MT3ΔNT	26.39 +/− 1.99	44.06 +/− 3.37	28.16 +/− 5.87	32.87
MT1E	32.68 +/− 7.26	35.92 +/− 6.98	30.91 +/− 8.41	33.17
MT1E-CT	22.96 +/− 11.94	29.34 +/− 9.40	37.98 +/− 7.07	30.09
MT1E-NT	37.58 +/− 10.23	34.3 +/− 9.4	32.28 +/− 12.61	34.72
MCF-7 (parent)	38.80 +/− 12.10	22.21 +/− 4.63	32.4 +/− 1.92	31.13
pcDNA 6.2/V5 Blank vector	41.99 +/− 5.38	27.38 +/− 10.76	36.80 +/− 6.48	35.39

Average TER for each mutant cell line was measured on days 5, 6 and 7 and is expressed as Ω/cm² +/− the SEM. The combined average TER for days 5, 6 and 7 was used to determine the relationship between TER and the presence or absence of the N- and C- terminal region of MT3. No statistical significance using the one-way ANOVA test and Dunnet’s post-test for multiple comparisons using the pcDNA 6.2/V5 cell line as control

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