Fig. 4

TanII A promoted autophagic body production in A375 cells. To assess the effects of TanIIA on autophagic body production in A375 cells, A375 cells were incubated with TanIIA at (0, 1.0, 2.0, or 4.0 μg/mL) respectively for 48 h. The cells were stained with acridine orange for flow cytometry analysis to monitor autophagic body production. a. TanIIA enhanced autophagic body production on A375 in a dose dependent manner. Each treatment was repeated three times (n = 3). *P < 0.05, ** P < 0.01, and ***P < 0.001 for comparison of indicated Tan II A treatments to the negative control (0 μg/mL Tan II A) respectively. b–e. The representative flow cytometry histograms of autophagic body staining on A375 cells treated with 0, 1, 2, or 4 μg/mL Tan II A respectively