Fig. 3
From: Linking hypoxia, DNA damage and proliferation in multicellular tumor spheroids
Proliferation and apoptosis in A673 spheroids. a Representative images showing the proliferative marker Ki-67 (red, with blue Hoechst 33,342 counterstain) in A673 spheroids <500 μm, 550–650 μm and >750 μm in diameter. b Representative images showing EdU incorporation (green, with blue Hoechst 33,342 counterstain) in A673 spheroids <500 μm, 550–650 μm and >750 μm in diameter. These are adjacent sections from the same spheroids imaged in Fig. 3a. c Cell death in A673 spheroids of <500 μm, 550–650 μm and >750 μm in diameter indicated by staining for cleaved caspase-3 (green, with blue Hoechst 33,342 counterstain)