Fig. 2
From: Linking hypoxia, DNA damage and proliferation in multicellular tumor spheroids
Hypoxia development and glycogen accumulation in growing spheroids. a Immunostaining of A673 and LLC spheroid cryo-sections for the hypoxia marker pimonidazole (red) shows hypoxia development at different sizes in the central core of A673 and LLC spheroids. Spheroids sections counter-stained with Hoechst 33,342. b Periodic Acid Schiff’s Base staining (dark pink) demonstrating glycogen storage in MCTS cryo-sections obtained from A673 spheroids of different sizes