Table 4 KRAS mutation analysis in single CTCs and primary tissue
Pt ID | Blood Sample ID | CTC | Primary tumor | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
PCR | WGA | |||||||||||||
Number of samples analyzed | Number of successful sequences | Sequence success rate (%) | KRAS status | Number of samples analyzed | Number of successful sequences | Sequence success rate (%) | KRAS status | |||||||
Number of wild-type cells | Number of mutant cells | Number of wild-type cells | Number of mutant cells | |||||||||||
I | #43 | 12 | 12 | 100% | 12 | 0 | wild | 9 | 7 | 77.8% | 7 | 0 | wild | wild |
#46 | 12 | 12 | 100% | 12 | 0 | wild | 18 | 16 | 88.9% | 16 | 0 | wild | ||
II | #44 | 2 | 2 | 100% | 2 | 0 | wild | - | - | - | - | - | - | wild |
III | #82 | 3 | 3 | 100% | 1 | 2 | p.G12A | - | - | - | - | - | p.G12A | |
VI | #92 | 10 | 7 | 70% | 7 | 0 | wild | - | - | - | - | - | - | wild |
#96 | 12 | 8 | 66.7% | 8 | 0 | wild | 1 | 1 | 100% | 1 | 0 | wild | ||
V | #95 | - | - | - | - | - | - | 6 | 6 | 100% | 6 | 0 | wild | wild |
#97 | 10 | 6 | 60% | 6 | 0 | wild | 16 | 14 | 87.5% | 14 | 0 | wild | ||
#142 | - | - | - | - | - | - | 4 | 4 | 100% | 4 | 0 | wild | ||
VI | #98 | 10 | 5 | 50% | 3 | 2 | p.G12D | 8 | 2 | 25% | 2 | 0 | wild | p.G12D |
#99 | 12 | 8 | 66.7% | 4 | 4 | p.G12D | 74 | 73 | 98.6% | 70 | 3 | p.G12D | ||
VII | #114 | 5 | 1 | 20% | 1 | 0 | wild | 5 | 3 | 60% | 3 | 0 | wild | wild |
VIII | #126 | - | - | - | - | - | - | 4 | 3 | 75% | 3 | 0 | wild | wild |
#129 | - | - | - | - | - | - | 7 | 6 | 85.7% | 6 | 0 | wild | ||
IX | #130 | 6 | 5 | 83.3% | 5 | 0 | wild | 8 | 8 | 100% | 7 | 1 | p.G13D | wild |
#131 | 6 | 2 | 33.3% | 1 | 1 | p.G13D | 13 | 7 | 53.8% | 6 | 1 | p.G12D | ||
X | #135 | - | - | - | - | - | - | 2 | 2 | 100% | 2 | 0 | wild | wild |
XI | #137 | 7 | 6 | 85.7% | 5 | 1 | p.G12D | 2 | 1 | 50% | 1 | 0 | wild | p.G12D |
Total | 107 | 77 | 72% | 177 | 153 | 85.9% | ||||||||