Fig. 4
From: Gamma-glutamyltransferase activity in exosomes as a potential marker for prostate cancer
GGT activity and GGT1 expression in exosomes isolated from human serum by SEC. Serum of a healthy individual (500 μL) was subjected to SEC. a CD9 expression in each fraction was measured by a sandwich ELISA. GGT activity in each fraction was determined by incubation with gGlu-HMRG at room temperature for 1 h and measurement of fluorescence intensity (Ex/Em 490/520 nm) using microplate reader. b The fractions 3–10 collected from a healthy individual were subjected to Western blot analysis for GGT1 large and small subunits and CD9. The upper band of the doublet corresponds to the GGT1 small subunit (shown by arrow)