Skip to main content

Table 1 Cell cycle changes caused by metformin and/or nutlin-3a

From: Metformin produces growth inhibitory effects in combination with nutlin-3a on malignant mesothelioma through a cross-talk between mTOR and p53 pathways

Cells Time (hrs) Treatment Cell cycle distribution (%)(Average ± SE)
Sub-G1 G1 S G2/M
MSTO-211H 24 (-) 4.35 ± 0.22 56.30 ± 0.35 17.93 ± 0.04 20.88 ± 0.44
  Met 20mM 5.43 ± 0.23 68.82 ± 0.25 12.91 ± 0.22 12.39 ± 0.23
  Met 60 mM 10.92 ± 0.63 63.25 ± 1.75 4.71 ± 0.16 20.88 ± 2.13
  Nut 10 μM 5.95 ± 0.11 84.18 ± 0.43 2.35 ± 0.16 7.32 ± 0.23
  Met 20mM
+ Nut 10 μM
7.39 ± 0.14 72.91 ± 0.29 3.00 ± 0.14 16.23 ± 0.29
  Met 60 mM
+ Nut 10 μM
10.56 ± 1.42 61.41 ± 1.50 7.93 ± 0.69 19.71 ± 0.71
48 (-) 0.76 ± 0.03 68.87 ± 0.65 12.70 ± 0.59 17.31 ± 0.20
  Met 20mM 3.40 ± 0.06 70.64 ± 0.26 11.96 ± 0.06 13.43 ± 0.21
  Met 60 mM 7.44 ± 0.12* 68.10 ± 0.30 5.29 ± 0.08 18.84 ± 0.13
  Nut 10 μM 6.11 ± 0.22* 85.90 ± 0.25 1.89 ± 0.03 5.92 ± 0.13
  Met 20mM
+ Nut 10 μM
6.51 ± 0.16 76.44 ± 0.24 2.82 ± 0.08 13.87 ± 0.18
  Met 60 mM
+ Nut 10 μM
11.34 ± 0.22* 63.25 ± 0.24 2.97 ± 0.04 22.25 ± 0.22
72 (-) 1.15 ± 0.11 82.29 ± 0.39 4.04 ± 0.17 12.26 ± 0.18
  Met 20mM 3.08 ± 0.09 76.19 ± 0.21 8.07 ± 0.14 12.25 ± 0.42
  Met 60 mM 13.28 ± 0.27* 65.29 ± 1.07 4.99 ± 0.10 16.24 ± 0.71
  Nut 10 μM 11.38 ± 0.14* 81.30 ± 0.28 1.88 ± 0.10 5.29 ± 0.13
  Met 20mM
+ Nut 10 μM
7.39 ± 0.15 75.38 ± 0.21 2.63 ± 0.06 14.26 ± 0.23
  Met 60 mM
+ Nut 10 μM
36.25 ± 0.44* 44.02 ± 0.25 3.28 ± 0.06 16.15 ± 0.26
NCI-H28 24 (-) 0.47 ± 0.04 60.27 ± 0.19 16.66 ± 0.34 22.06 ± 0.25
  Met 20mM 0.43 ± 0.02 67.39 ± 0.41 11.76 ± 0.29 19.66 ± 0.13
  Met 60 mM 0.89 ± 0.08 44.81 ± 0.07 15.01 ± 0.17 38.33 ± 0.17
  Nut 10 μM 0.77 ± 0.02 40.63 ± 0.20 9.25 ± 0.42 47.61 ± 0.36
  Nut 40 μM 1.22 ± 0.07 44.87 ± 0.57 15.17 ± 0.57 37.12 ± 0.65
  Met 20mM
+ Nut 10 μM
1.25 ± 0.04 42.33 ± 0.36 13.30 ± 0.38 41.28 ± 0.06
  Met 60 mM
+ Nut 40 μM
1.24 ± 0.15 48.20 ± 0.37 20.92 ± 0.17 28.35 ± 0.38
48 (-) 1.54 ± 0.05 60.08 ± 0.39 16.04 ± 0.36 21.88 ± 0.01
  Met 20mM 0.45 ± 0.04 73.97 ± 0.18 6.35 ± 0.05 18.66 ± 0.10
  Met 60 mM 1.06 ± 0.10 40.68 ± 0.08 14.65 ± 0.19 42.61 ± 0.19
  Nut 10 μM 3.84 ± 0.10 43.82 ± 0.29 8.28 ± 0.20 42.47 ± 0.26
  Nut 40 μM 2.50 ± 0.10 49.77 ± 0.22 10.35 ± 0.10 36.08 ± 0.21
  Met 20mM
+ Nut 10 μM
0.91 ± 0.09 46.45 ± 0.74 9.23 ± 0.29 41.90 ± 0.90
  Met 60 mM
+ Nut 40 μM
1.38 ± 0.12 49.24 ± 0.21 20.16 ± 0.30 27.63 ± 0.15
72 (-) 1.49 ± 0.02 64.68 ± 0.34 15.58 ± 0.03 17.79 ± 0.30
  Met 20mM 0.52 ± 0.09 75.59 ± 0.14 4.52 ± 0.08 18.86 ± 0.21
  Met 60 mM 1.50 ± 0.31 41.66 ± 0.37 15.31 ± 0.03 40.59 ± 0.27
  Nut 10 μM 6.09 ± 0.20 43.98 ± 0.25 8.75 ± 0.23 39.57 ± 0.41
  Nut 40 μM 8.96 ± 0.83 61.92 ± 0.79 7.31 ± 0.02 21.05 ± 0.49
  Met 20mM
+ Nut 10 μM
1.01 ± 0.28 46.14 ± 0.66 9.14 ± 0.11 42.20 ± 0.41
  Met 60 mM
+ Nut 40 μM
9.00 ± 0.72 44.61 ± 0.43 15.83 ± 0.25 29.48 ± 0.45
EHMES-10 24 (-) 0.46 ± 0.05 70.86 ± 0.69 9.64 ± 0.3 18.63 ± 0.57
  Met 20mM 1.04 ± 0.06 73.58 ± 0.31 8.78 ± 0.05 16.18 ± 0.32
  Met 60 mM 1.61 ± 0.06 67.93 ± 0.31 6.95 ± 0.14 23.13 ± 0.28
  Nut 20 μM 1.19 ± 0.10 73.47 ± 0.21 10.38 ± 0.26 14.38 ± 0.29
  Nut 60 μM 1.19 ± 0.08 80.17 ± 0.16 3.49 ± 0.06 14.74 ± 0.05
  Met 20mM
+ Nut 20 μM
0.85 ± 0.02 76.79 ± 0.23 7.35 ± 0.09 14.61 ± 0.19
  Met 60 mM
+ Nut 60 μM
2.37 ± 0.19 73.06 ± 0.38 5.2 ± 0.38 18.93 ± 0.32
48 (-) 0.92 ± 0.04 73.14 ± 0.06 10.34 ± 0.23 15.22 ± 0.35
  Met 20mM 1.46 ± 0.13 61.87 ± 0.16 13.00 ± 0.12 23.04 ± 0.21
  Met 60 mM 2.67 ± 0.12* 66.72 ± 0.12 9.32 ± 0.11 20.73 ± 0.13
  Nut 20 μM 1.62 ± 0.20 67.78 ± 0.21 12.12 ± 0.17 18.02 ± 0.17
  Nut 60 μM 1.85 ± 0.16* 84.44 ± 0.12 3.32 ± 0.05 10.15 ± 0.06
  Met 20mM
+ Nut 20 μM
1.35 ± 0.04 66.71 ± 0.35 13.01 ± 0.32 18.19 ± 0.42
  Met 60 mM
+ Nut 60 μM
4.57 ± 0.17* 74.63 ± 0.17 5.44 ± 0.16 15.11 ± 0.2
72 (-) 2.18 ± 0.55 56.86 ± 0.62 12.2 ± 0.75 27.79 ± 1.57
  Met 20mM 1.89 ± 0.11 63.77 ± 0.19 11.90 ± 0.21 21.64 ± 0.42
  Met 60 mM 3.07 ± 0.14* 64.22 ± 0.13 8.22 ± 0.12 23.99 ± 0.22
  Nut 20 μM 0.73 ± 0.03 67.46 ± 0.28 11.05 ± 0.19 20.22 ± 0.39
  Nut 60 μM 3.47 ± 0.11* 84.27 ± 0.25 3.4 ± 0.17 8.74 ± 0.07
  Met 20mM
+ Nut 20 μM
1.27 ± 0.03 70.85 ± 0.23 9.44 ± 0.43 17.85 ± 0.19
  Met 60 mM
+ Nut 60 μM
13.85 ± 0.86* 62.12 ± 0.26 6.35 ± 0.25 17.57 ± 0.86
  1. *P < 0.05, compared between combination and either metformin or nutlin-3a single treatment. N = 3
  2. Met Metformin, Nut Nutlin-3a