Fig. 3

GLA attenuates the expression/activation of Wnt/β-catenin of breast cancer cells through miR-148a. a-d MDA-MB-231 or Hs-578 T cells were pre-transfected by anti-miR-negative control or anti-miR-148a for 12 h, and then treated with 20 μM GLA for 48 h. a qRT-PCR analyses of miR-148a (mean ± SD, n = 3). b Western blot analysis relative protein levels of Wnt1 and non-phospho (active) β-catenin. c–d Expression of Wnt1 and TCF/LEF were analyzed by qRT-PCR (mean ± SD, n = 3). ^* P < 0.05 and ^** P < 0.01 compared with the anti-miR-negative control. ^# P < 0.05 compared with the cells treated with GLA and anti-miR-negative control