Fig. 3

Arsenic exposure caused multiple centrosome formation. a E7 cells were treated with arsenic (1 μM) for one week. These cells were fixed and labeled with Aurora-A and α-tubulin antibodies conjugated with FITC (Aurora-A) and PE (α-tubulin), respectively, and finally imaged under confocal microscopy. The Hoechst was used as nuclear counterstain. b E7 cells were treated with or without arsenic (1 μM) for one, two, and four weeks, then fixed and labeled as the same as panel (a). Quantification of the numbers of multiple centrosomes was normalized by mitotic cells (Aurora-A positive cells). Results are represented in two independent experiments. Student’s t test was used, *p < 0.05, **p < 0.01