Fig. 4
Effects of 1000 μmol/l and 1500 μmol/l 2250 on viability, apoptosis and necrosis in different malignant pancreatic cell lines measured by FACS analysis. AsPC-1 (a), BxPC-3 (b) and Panc-TuI (c) cells were incubated with 2250 (1000 and 1500 μmol/l) and ddH2O (control) for 24 h. The percentages of viable, apoptotic and necrotic cells were determined by FACS-analysis with Annexin V-FITC and Propidiumiodide. Values are means ± SEM of 4–6 independent experiments with three consecutive passages. Asterisk symbols on columns indicate differences between control and 2250 treatment. *** p ≤ 0.001, ** p ≤ 0.01, * p ≤ 0.05, n.s. p > 0.05 (one-way ANOVA followed by Tukey’s post-hoc test)