Fig. 5

Effect of fatty acids on cell proliferation. a Docosatetraenoic acid or EPA concentration of TUHR14TKB cells transfected with the FABP7 or lacZ expression vector. Four independent cell cultures were harvested, and the concentration of docosatetraenoic acid or EPA was determined. The data represent the average and standard deviation. b Cell proliferation was assayed in the presence or absence of 50 μM docosatetraenoic acid or EPA in RPMI 1640 medium containing 10% FBS, 5 mg/L blasticidin S HCl, 0.3 g/L G418, and 1 mg/L doxycycline hyclate. The doubling times of TUHR14TKB lacZ cells were determined using an MTS assay. The data represent the average and standard deviation (error bars) of 12 experiments