Fig. 1
From: u-PAR expression in cancer associated fibroblast: new acquisitions in multiple myeloma progression
a Flow cytometry analysis of CAF frequency (αSMA/FSP-1 positive cells on gated CD45- population) in patients with MGUS, aMM, rMM and sMM. Data are reported as mean ± SD from three different experiments performed in triplicate (*p < 0.01). On the right, representative flow cytometry histograms of FSP-1 positive cells for each study group. b Quantitative PCR analysis of α-SMA, Vimentin and MMP-2 in CAF. Results were obtained in 3 different experiments performed in triplicate. c Representative immunofluorescence panels of α-SMA (red) and Vimentin (red) in CAF from MGUSand MM patients at different disease phase (600X magnification)