Fig. 2

Endothelial Dll4 over-expression suppresses chemically- induced skin tumor onset and development. D4BE and D4OE mice were treated topically with a single dose of DMBA followed by two weekly applications of TPA for 19 weeks. a Representative pictures of D4BE and D4OE mice taken at the experiment end- point, i.e. 20 weeks after the DMBA- initiation, illustrate tumor-suppressing effect of endothelial Dll4 over-expression. b Percentage of tumor-bearing mice, number of tumors per mouse (c), mean tumor volume (d) and over-all tumor burden per mouse (e), calculated as the sum of tumor volumes developed by a mouse, throughout the tumor TPA-promotion in D4BE vs. D4OE mice. Error bars represent SEM. *, P < 0.05 and **, P < 0.01 were considered statistically significant and highly significant, respectively. Results are representative of n = 10 per mice group