Fig. 4
From: Effects of the lysosomal destabilizing drug siramesine on glioblastoma in vitro and in vivo
Propidium iodide uptake in flat surface spheroid migration assay. The glioblastoma stem cell-like containing spheroid (GSS) culture T78 were allowed to migrate for 1 day before exposure to 10 μM siramesine, while being incubated with the dye propidium iodide (PI), which enters dead and dying cells. a–i In control spheroids, there was a weak central PI uptake but no uptake was observed in the migrating cells. j–l In siramesine-exposed spheroids, PI uptake suggested siramesine-induced cell death in the central spheroid itself but also in the migrating cells already 6 h after exposure. m–r After 24 and 48 h of exposure a pronounced PI uptake was found both in the spheroid and in the migrating cells. Control cells received culture medium or DMSO (images not shown) both without siramesine. Scalebar 100 μm