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Fig. 1 | BMC Cancer

Fig. 1

From: A novel FOXO1-mediated dedifferentiation blocking role for DKK3 in adrenocortical carcinogenesis

Fig. 1

Reduced DKK3 expression in ACC. a Reduced DKK3 gene expression in 7 tumor samples (T1–T7) compared to 3 histologically normal adrenal samples (N1–N3). T5a and T5b: RNA from two different areas of one tumor. Magnitude of gene expression relative to housekeeping gene panel shown below. b Immunofluorescence detection of DKK3 and β-catenin in normal adrenal cortex (a-g) and ACC (h-n). Tissue sections treated with primary/secondary antibodies for DKK3 (FITC, green; c, j) or β-catenin (TR, red; e, l), DAPI (blue for nuclear staining; b, i), or combinations of FITC/DAPI (d, k), TR/DAPI (f, m), or FITC/TR/DAPI (a, h, g, n). a and h: 100× magnification; b-g, i-n: 400× magnification; inset (g, n): 1000× magnification. c DKK3 gene expression (fold-change) in 37 ACC samples relative to average expression of 14 normal adrenal samples normalized to 1. d Average DKK3 expression (fold-change) in study cohort (n = 37) compared to average expression from 14 normal adrenal samples

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