Table 3 Discrepancies between cobas PCR for BRAF and NGS. As cobas does not distinguish mutations at BRAF codon 600, these are designated V600X in the table, WT is wild-type
From: Validation of an NGS mutation detection panel for melanoma
Case (Sample) | cobas | NGS | Comment | True discrepancy |
|---|---|---|---|---|
30 | WT | BRAF | Mutation not present on cobas | Yes |
33 | WT | BRAF | Mutation not present on cobas | Yes |
35 | WT | BRAF | Mutation not present on cobas | Yes |
48 | V600X | MEK1 | Resistance gene due to vemurafenib treatment | No |
85 | V600X | NRAS | Resistance gene due to vemurafenib treatment | No |
100 | V600X | KIT | Resistance gene due to vemurafenib treatment | No |