Fig. 5
From: Genome-independent hypoxic repression of estrogen receptor alpha in breast cancer cells
Hypoxic repression of ER activity inhibits proliferation. In vitro growth curves of cells grown with or without estradiol (E2) and at normoxia or hypoxia (1% O2). a MDA-MB-231, b MDA-MB-453, c MCF7, d BT474, e T47D and f ZR75B. Dashed lines are estrogen-free media, whereas solid lines contain 10 nM of estradiol. Black lines represent normoxic conditions, whereas gray lines are cells under hypoxic conditions. Two-way ANOVA analysis: a p > 0.05 MDA-MB-231, b p > 0.05 MDA-MB-453, c *p < 0.001 MCF7, d *p < 0.001 BT474, e *p < 0.001 T47D, f *p < 0.001 ZR75B. g Comparison of cell cycle distributions for cultures grown for 3 days at normoxia or hypoxia (1% O2). (T-student test *p < 0.001 MCF7, *p < 0.001 BT474, *p = 0.001 T47D, *p < 0.001 ZR75B). BT474 analysis was at a 6 day time point