Fig. 2
From: Genome-independent hypoxic repression of estrogen receptor alpha in breast cancer cells
Hypoxic repression of ER-α is dependent on HIF-1α. a Representative western blots of HIF-1α, HIF-2α and β-actin protein in ten ER-positive cell lines grown at normoxia or hypoxia (1% O2, 24 h). b qPCR analysis of HIF1A mRNA levels in MCF7, BT474, T47D and ZR75B transfected with shScramble or shHIF1A. Relative HIF1A mRNA levels normalized to TBP. (Change in HIF1A levels: *p < 0.0001 MCF7, *p = 0.012 BT474, *p < 0.0001 T47D, *p = 0.0046 ZR75B). c Representative western blots of HIF-1α, ER-α and β-actin protein from MCF7, BT474, T47D and ZR75B with either shScramble or shHIF1A at normoxia and hypoxia (1% O2, 24 h). β-actin is used as a loading control. d Representative western blot of HIF-1α, ER-α and β-actin protein from MCF7, BT474, T47D and ZR75B with or without the transfection of stabilized HIF-1α (HIF-1αODD)