Fig. 5

RhoBTB1 is expression is downregulated in breast cancer cell lines. a-d The expression of RhoBTB1, RhoBTB2, METTL7A and METTL7B was measured by RT-PCR in three well-characterized breast cancer cell lines: MDA-MB-231, T47D and MCF7. Expression was compared with two well-characterized normal mammary epithelial cell lines: MCF-10A and HMT-S1. Data are means ± SEM (n = 3). Data were normalized to HeLa cell samples. Comparisons are to the MCF10A control; *P <0.05; **P <0.01, ***P <0.001, ****P <0.0001. All three breast cancer lines showed a markedly reduced RhoBTB1 expression, as well as reduction in expression of METTL7A and METTL7B. T47D cells had the lowest levels of both RhoBTB1 and RhoBTB2. e the breast cancer cell lines were fixed and stained for giantin (green) and Golgi morphology compared to the normal mammary epithelial HMT-S1 line. T47D cells showed marked fragmentation of the Golgi. Bar = 10 μm