Fig. 2From: Testis specific Y-like 5: gene expression, methylation and implications for drug sensitivity in prostate carcinomaReactivation of TSPYL5 mRNA and expression of DNMT’s in PC cells. a Induction of TSPYL5 mRNA expression in DU145 and LNCaP cells upon treatment with DT (0.5 μM) for 4 days at 37 °C. Significant induction of TSPYL5 mRNA was observed both in LNCaP (P = 0.001*) and DU145 cells (P = 0.0021**). b RT-PCR for DNMT1 and DNMT3B mRNA and immunoblot analysis for protein expression in nuclear fractions of the PC cells. Histone-H3 was used as a housekeeping proteinBack to article page