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Fig. 6 | BMC Cancer

Fig. 6

From: Up-regulation of miR-146a increases the sensitivity of non-small cell lung cancer to DDP by downregulating cyclin J

Fig. 6

Overexpression of miR-146a and knockdown of CCNJ further enhanced the sensitivity to DDP in A549/DDP and SPC-A1/DDP cells. a The cell vitality was evaluated by CCK-8 assay, A549/DDP/miR-146a and SPC-A1/DDP/miR-146a cells transfected with siCCNJ or NC for 6 h and then treated with serially diluted DDP. b Representative data of FACS statistical graph analyses of cell cycle in A549/DDP/miR-146a and SPC-A1/DDP/miR-146a cells transfected with siCCNJ or NC for 6 h and then incubated with 1 μg/ml DDP for 48 h. c Representative data of FACS and statistical graph analyses of cell apoptosis in A549/DDP/miR-146a and SPC-A1/DDP/miR-146a cells transfected with siCCNJ or NC for 6 h and then incubated with 1 μg/ml DDP for 48 h. d Representative data of Hoechst staining assay inA549/DDP/miR-146a and SPC-A1/DDP/miR-146a cells transfected with siCCNJ or NC for 6 h and then incubated with 1 μg/ml DDP for 48 h. All data were expressed as mean value ± SD from 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001

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