Fig. 6

Interactome of WDR13. a Interaction of FLAG-tagged WDR13 with endogenous NCoR1, SMRT and c-Jun in HEK293 cells. WDR13 interacts with endogenous NCoR1, c-Jun but not with SMRT. Arrows show specific band. b Co-immunoprecipitation of Myc-Wdr13 and various HDACs show interaction of WDR13 with HDAC1 and HDAC3 in HEK293 cells. 5% input shows protein expression in cell lysate with Myc-specific antibody in the lower panels. c Interaction of β-catenin and NFκB with various deletion constructs of FLAG-Wdr13 shows absence of evidence of any interactions in HEK293 cells. Domain organization of WDR13 and schematic of various deletion constructs used in these immunoprecipitation experiments are mentioned in Fig. 5c