Fig. 4
WDR13 activates c-Jun activity in JNK-dependent manner. a Levels of p53 in Wdr13 +/0 and Wdr13 -/0 MEFs before and 24 h after UV treatment (40 J/m2). Actin was used as loading control. b Effect of UV on the expression of p-c-Jun, t-c-Jun, p-JNK, t-JNK and WDR13 in Wdr13 +/0 and Wdr13 -/0 MEFs. Actin was used as loading control. Arrows show specific band. c c-Jun promoter-reporter activity in UV untreated and treated (40 J/m2) Wdr13 +/0, Wdr13 -/0 and MEFs – over-expressing WDR13 (c-Jun-Luc- 350 ng, SV40-RL-100 ng, pGFP-150 ng or pCMV-FLAG-Wdr13-150 ng). d c-Jun promoter-reporter activity in anisomysin untreated and treated (16 h) Wdr13 +/0, Wdr13 -/0 and MEFs – over-expressing WDR13 (c-Jun-Luc- 350 ng, β-gal-100 ng, pGFP-150 ng or pCMV-FLAG-Wdr13-150 ng). For panel C and D, Black bar, Wdr13 +/0 MEFs; white bar, Wdr13 -/0 MEFs; Light grey bar, MEFs over-expressing WDR13;. e p-JNK and t-JNK levels in HEK293, MCF7 and HT29 cells before UV treatment and 2 h after UV treatment. f c-Jun promoter-reporter activity in HT29 cells shows activation of reporter after Wdr13 over-expression in dose-dependent manner (c-Jun-Luc-250 ng, SV40-RL-100 ng, pGFP-250 ng, 200 ng, 150 ng, 100 ng, and pCMV-FLAG-Wdr13-0 ng, 50 ng, 100 ng, 150 ng). g c-Jun promoter-reporter activity in COLO205 cells shows activation of reporter after Wdr13 over-expression (c-Jun-Luc-350 ng, SV40-RL-50 ng and pGFP-200 ng or pCMV-FLAG-Wdr13-200 ng). All transfections were performed in technical triplicates. Student’s t-test was used for statistical analysis and error bar shows s.e.m