Fig. 3
From: Gastrin activates autophagy and increases migration and survival of gastric adenocarcinoma cells
Gastrin induced migration is dependent on autophagy. (a) AGS-Gr cells treated with gastrin (1 nM), BafA1 (100 nM) and YM022 (50 nM). Migration was monitored real-time for 24 h using xCELLigence technology. Untreated (blue), gastrin (green), YM022 (pink), gastrin + YM022 (violet), BafA1 (red), gastrin + BafA1 (light blue). Graphs represent of one of three independent experiments. (b) AGS-Gr cells treated with gastrin, YM022 and HCQ (20 μM)). Untreated (violet), Gastrin (light blue), HCQ (pink), HCQ + gastrin (dark blue), HCQ + YM022 (green), HCQ + YM022 + gastrin (red). (c) Gastrin induced migration is dependent on ULK1. AGS-Gr cells were treated with gastrin and ULK1 inhibitor SBI-026965 (SBI) (10 μM) Untreated (red), gastrin (green), SBI-026965 (blue), gastrin + SBI-026965 (pink). (a, b and c) Graphs represent of one of three independent experiments. Bar graphs represent mean +/− SEM (n = 3, P-value*: ≤ 0.05, ** ≤ 0.01, *** ≤ 0.001)