Fig. 2
From: Gastrin activates autophagy and increases migration and survival of gastric adenocarcinoma cells
Gastrin induced autophagy is mediated via the CCKBR: (a and b) AGS-Gr and MKN45 pretreated overnight with YM022 (100 nM) before treatment with BafA1 and gastrin for 4 h. The expression of MAP1LC3B-II and SQSTM1 is shown by immunoblots representing one of three independent experiments. (c) MKN45 cells transfected with siRNA CCKBR. Protein expression of CCKBR was analyzed by immunoblotting. (d) MKN45 cells transfected with siRNA CCKBR and treated with BafA1 and gastrin. MAP1LC3B-II and SQSTM1 expression is shown by immunoblotting. The image represents one of three independent experiments. Bar graphs (a, b, c and d) show mean +/− SEM, (n = 3, P- value ** ≤ 0.02 and * ≤ 0.05)