Fig. 2

Osteosarcoma cells migration to lung cell CM were further increased in the presence of lung cells that were placed in the bottom chamber of the transwell. a 160 μl of HULEC-5a CM, HULEC-5a CM containing 1x10⁴ HULEC cell, and HULEC-5a CM containing 5x10⁴ HULEC cell were loaded onto the bottom well of CIM Plate 16. 2x10⁴ SaOS2 or SJSA-1 cells in 100 μl serum free medium were added to the top well of CIM Plate 16. OS cell migration index was calculated every 15 min for 100 reading. Each plate had negative controls where no cells were added to the top well and either serum free media or FBS was added to the bottom well. b 160 μl of HULEC-5a CM, HULEC-5a CM containing 1x10⁴ HULEC cell, and HULEC-5a CM containing 5x10⁴ HULEC cell were placed in the bottom chamber of the transwell and compared to MRC5 CM, MRC5 CM containing 1x10⁴ MRC5 cell and MRC5 CM containing 5x10⁴ MRC5 loaded onto the bottom well of CIM-Plate 16. 2x10⁴ SJSA-1 cells in 100 μl serum free medium were added to top well of CIM Plate 16, and SJSA-1 cell migration index was calculated every 15 min for 100 reading. Each plate had negative controls where no cells were added to the top well and either serum free media or FBS was added to the bottom well. Serum free media in the bottom well was also tested with SJSA cells