Fig. 6From: Norcantharidin Inhibits cell growth by suppressing the expression and phosphorylation of both EGFR and c-Met in human colon cancer cellsEffects of NCTD on the key proteins regulating cell cycle and apoptosis in HCT116 (a) and HT29 (b) human colon cancer cells. The cells were seeded in 10-cm dishes for 24 h and then treated with different concentrations of NCTD. After 72 h of incubation, cells were collected for western blot analysis as described in the Materials and Methods. The numbers underneath of the blots represent band intensity (normalized to β-Actin, means of three independent experiments) measured by Image J software. The standard deviations (all within ± 15% of the means) were not shown. β-Actin was served as an equal loading control. The experiments were repeated for three timesBack to article page