Fig. 4

Cell cycle distribution of HCT116 (a) and HT29 (b) human colon cancer cells after treatment with NCTD. The cells were seeded in 6-well plates for 24 h and then treated with NCTD in complete media. After 24 h treatment, cells were harvested and subjected to cell cycle analyses as described in Materials and Methods. All data represent mean ± SD. Statistical analysis were conducted among control and treated groups in G0/G1, S and G2/M phases separately. Statistical significance was determined by Student’s t test (p < 0.001). * represent significant difference in cell number in the control that received PBS versus those treated with the indicated concentration of NCTD. Each bar represents the average value ± S.D; n = 3