Fig. 5
From: Cryptotanshinone activates AMPK-TSC2 axis leading to inhibition of mTORC1 signaling in cancer cells
Inhibition of AMPK with Compound C attenuates CPT-induced inhibition of mTORC1 and cell growth. a Serum-starved Rh30 cells were firstly exposed to Compound C (10 μmol/L) for 0.5 h, and then treated with CPT (0-20 μmol/L) for 2 h, finally stimulated with IGF-1 (10 ng/mL) for 1 h, followed by western blotting assay for proteins expression. b Serum-starved Rh30 cells were firstly exposed to Compound C (10 μmol/L) for 0.5 h, then treated with CPT (0-20 μmol/L) for 48 h, followed by taking photograph using Image system