Fig. 2

CUBN antibody validation. a Two antibodies targeting the CUBN protein at different epitopes (HPA043854 and HPA004133) were tested using immunohistochemistry on a range of normal and malignant tissue. Included in this figure are staining examples from normal human kidney (K) and two renal cell carcinoma cases (RCC1 and RCC2). As chromogen 3,3’-Diaminobenzidine (DAB) was used. b RNA-seq expression data from normal human kidney (K) and the renal cell carcinoma cases (RCC1 and RCC2). Expression levels are indicated as fragments per kilobase of exon model per million mapped reads (FPKM). c Western blot analysis of CUBN expression in protein extracts from normal human kidney and the renal cell carcinoma cases RCC1 and RCC2 using HPA043854 and HPA004133