Fig. 2

Representative photo-micrographs of RMLEC tubular network formation in response to different agents. a Tube formation were measured on GFR-Matrigel at 30 h in the presence of BSA(control), COX-1/2 inhibitor indomethacin (20μM), COX-2 selective inhibitor NS-398 (20μM), EP4 antagonist RQ-15986 (1μM), PGE2 (10μM), EP4 agonist PGE1-OH (10μM), and 24h C3L5-CM. Bars equal 100 μm. b Quantification of the number of branching points in the captured area representing 10 different fields of view, (FOV) for each experimental condition. Each bar graph represents each experimental condition. Error bars represent mean ± SEM of three different experiments. (**) represents p < 0.001