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Fig. 2 | BMC Cancer

Fig. 2

From: Protein kinase C inhibitor Gö6976 but not Gö6983 induces the reversion of E- to N-cadherin switch and metastatic phenotype in melanoma: identification of the role of protein kinase D1

Fig. 2

Gö6976 but not Gö6983 inhibits N-cadherin expression and induces E-cadherin expression in M2 metastatic melanoma cells. M2 metastatic melanoma cells were seeded in 6-well plates at the density of 25,000 cells per well. After three to five days of culture, cells were treated with 1 μM Gö6976 or 1 μM Gö6983 for different periods of time (0, 1, 3, 24 or 48 h). Cells were then lysed and proteins analyzed by western blot using anti-E-cadherin, anti-N-cadherin or anti-actin antibodies. The autoradiograms presented are those of a typical experiment. The histogram represents quantitative analysis of N-cadherin expression normalized to untreated control cells (0 h). The results presented are the means ± SD for three independent experiments. **, p < 0.01 versus untreated cells

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