Fig. 4
From: The miR-34a-5p promotes the multi-chemoresistance of osteosarcoma via repression of the AGTR1 gene
The effects of forced reversal of miR-34a-5p or AGTR1 levels on the chemoresistance of G-292 and SJSA-1 cells. a The mRNA level of AGTR1 detected by qRT-PCR in the 5PM-, or siRNA- versus the NC-transfected G-292 cells. b The levels of AGTR1 protein detected by Western analysis in the siRNA- versus the NC-transfected G-292 cells. c The IC50 dosed drug-triggered cell death of G-292 cells transfected with miR-34a-5p mimic (5PM) or the gene specific siRNAs versus the negative control (NC). d The level of AGTR1 by qRT-PCR in the 5PA, or GFP-AGTR1 versus the NC transfected SJSA-1 cells. e The levels of AGTR1 protein by Western analysis in the GFP-AGTR1 versus the NC transfected SJSA-1 cells. f The IC50 dosed drug-triggered cell death of SJSA-1 cells transfected by miR-34a-5p mimic (5PA) or GFP-AGTR1 versus the negative control (NC). g, h and i The effects of the forced reversal of both miR-34a-5p and AGTR1 levels on the apoptosis by FACS analysis of G-292 cells in plot and in the original. (*, P < 0.05)