Fig. 1

Immunotherapy of melanoma B16-F10 using MPLA alone or in combination with mannan-BAM. C57BL/6 mice (females) were inoculated subcutaneously in a shaved area of the right flank with 4 × 10⁵ murine melanoma B16-F10 cells per mouse in 0.1 ml RPMI. Twelve days after tumor transplantation, mice were randomized in groups of six. Therapies started immediately. The preparations were applied intratumorally (50 microliters/mouse) in pulse regime (days 0, 1, 2…8, 9, 10). After therapy commenced, mice were kept individually. Tumors were measured every second day for 14 days and their volume was calculated. The composition of preparations used was: 0.5 mg MPLA/ml PBS, 0.5 mg MPLA/ml 0.2 mM mannan-BAM in PBS, 0.2 mM mannan-BAM in PBS, PBS