Fig. 3

Apoptosis in MMTV-PyMT;Apc ^Min/+ and MMTV-PyMT;Apc ^+/+ cells treated with chemotherapeutic drugs and targeted inhibitors. a Apoptosis was measured by cleaved caspase 3 IF in the presence of cisplatin. Treatment with cisplatin and either PP2 or SP600125 significantly increases apoptosis compared to cisplatin alone in MMTV-PyMT;Apc ^Min/+cells. No effect was observed with the addition of PP2 or SP600125 in the MMTV-PyMT;Apc ^+/+cells. b Apoptosis was measured by cleaved caspase 3 IF with doxorubicin treatments. c Representative cleaved caspase 3 IF images of cells treated with cisplatin and the targeted inhibitor. The scale bar is equal to 200 microns and arrows are used to depict specific cleaved caspase 3 (CC 3) positive cells in each image. Data are shown as the means ± SEM from 3 independent experiments; *P < 0.05 when comparing MMTV-PyMT;Apc ^Min/+ to MMTV-PyMT;Apc ^+/+ cells and ** P < 0.05 when comparing the combination treatment versus a single agent (cisplatin or doxorubicin)