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Fig. 4 | BMC Cancer

Fig. 4

From: Synthesis and bio-molecular study of (+)-N-Acetyl-α-amino acid dehydroabietylamine derivative for the selective therapy of hepatocellular carcinoma

Fig. 4

a Comet assay for DNA breaks: DAAD2 (5 μM), adriamycine (1 μM), camptothecin (5 μM) and DMSO controls (up to 0.1%). Photographs were taken after 72 h; obvious alkaline comets are shown in Hep3B cells treated with adriamycine and camptothecin. b Annexin V/PI Staining: DAAD2 (2 & 5 μM), camptothecin (5 μM) and DMSO controls (up to 0.1%). Photographs were taken after 72 h. DAAD2 treated Hep3B cells showed the apoptotic mode of cell death. c Immunoperoxidase Assay: Compound DAAD2 (2 & 5 μM), camptothecin (5 μM) and DMSO controls (upto 0.1%). Photographs were taken after 48 h. DAAD2 (2 μM) treated cells showed weak activation of caspase-3 cleaved

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BMC Cancer

ISSN: 1471-2407

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