Fig. 2

Leptin exposure up-regulates miR-4443 and decreases invasion, while overexpression of miR-4443 decreases the proliferation of cultured HCT-116 cells. a. RT-qPCR validation of the effects of leptin and insulin, both at 200 ng/ml, on the expression of candidate miRNAs identified by Nanostring profiling, in HCT-116 cells. Bars, SD from triplicates. b Dose response of miR-4443 levels under leptin exposure, in HCT-116 cells. *:p < 0.05; t-test; bars: SD from triplicates. c CyQuant quantification of Matrigel invasion assay over 48 h, in HCT-116 cells with or without 100 ng/ml leptin. *:p < 0.05; t-test; bars: SE; n = 6. d CyQuant quantification of Matrigel invasion assay over 48 h, in HCT-116 cells transfected with miR-4443 mimic or negative control (miR-Neg). bars: SE; n = 5. e CyQuant quantification of cell proliferation over 48 h, in HCT-116 cells transfected with miR-4443 mimic or negative control (miR-Neg). Cell death-causing TOX oligo was used as transfection control. *:p < 0.05; t-test; bars: SE; n = 9