Fig. 8

a SCL2A1-GLUT1 gene expression increased under hypoxia by 3-BrPA is antagonized by Prima-1 in MelJuso cells . RT-qPCR was used to assay the effect of 3-BrPA and Prima-1 on SLC2A1-GLUT1 normalized to ACTN gene expression under normoxia or 3 h of hypoxia in MelJuso cells, treated as indicated in Fig. 4a. Note that combined use of Prima-1 and 3-BrPA lowers SLC2A1-GLUT1 expression induced by 3-BrPA after 3 h hypoxia in MelJuso cells. b 3-BrPA or Prima-1 preferentially decrease hypoxic SCL2A1-GLUT1 gene expression in C8161 cells. RT-qPCR was used to assay the effect of 3-BrPA and Prima-1 on SLC2A1-GLUT1 normalized to ACTN gene expression under 3 or 5 h of hypoxia in C8161 cells, treated as indicated in Fig. 4a. Here, Prima-1, 3-BrPA or both treatments lowered SLC2A1-GLUT1 gene expression. *denotes significance between treated cells relative to controls. ** denotes significance between unequal treatments. ***denotes significance between 3 h and 5 h hypoxia