Fig. 4

a Prima-1 lowers SLC2A1-GLUT1 gene expression in 5 mM glucose. A549 cells exposed to 5 mM glucose, 2 mM glutamine and 5% dialyzed serum, were kept for 4 h in hypoxia whenever indicated, followed by RNA isolation, and RT-qPCR, to assay SLC2A1-GLUT1 and ACTN gene expression bv qPCR, as indicated under Methods. b Decrease in ALDH1A1 induced by 3-BrPA is potentiated by Prima-1. Cells treated as indicated above were exposed to hypoxia whenever indicated, followed by RNA isolation, and RT-qPCR, to assay ALDH1A1 and ACTN gene expression bv RT- qPCR. c 3-BrPA counteracts Prima-1 hypoxic induction of VEGF gene expression. Cells treated as indicated above were exposed to hypoxia whenever indicated, followed by RNA isolation, and RT-qPCR, to assay VEGF and ACTN gene expression by RT- qPCR. d 3-BrPA suppresses CAIX gene expression. Cells seeded as indicated above were exposed to hypoxia for 4 h whenever indicated, followed by RNA isolation, and RT-qPCR, to assay CAIX and ACTN gene expression by q PCR. *denotes significance between treated cells relative to controls. **denotes significance between unequal treatments. ***denotes significance between hypoxia and normoxia