Fig. 4

Par3 regulates IL-6/STAT3 pathway. a Effect of siPar3 on STAT3 expression. JHOC cells were transfected with siPAR3 or control siRNA (siControl). Total cell extracts were then obtained after 48 h, and phospho-STAT3, total STAT3, and α-Tubulin proteins were detected by western blotting. The right-hand histogram shows the relative intensities of the blots of pSTAT3 normalized to α-Tubulin. Data are the mean (±SEM) of three independent experiments. b Effect of siPar3 on IL-6 secretion. JHOC5 cells were transfected with siPar3 or control siRNA for 48 h, then conditioned media were collected for ELISA analysis of IL-6. Data are the mean (±SEM) of three independent experiments. c Effect of Par3 overexpression on STAT3 expression. JHOC5 cells were transfected with pcDNA 3.1 (empty vector) or myc-Par3 for 24 h and Par3, phospho-STAT, total STAT, and α-Tubulin levels were analyzed by western blotting