Fig. 7

Combinatorial treatments reduce colony-forming ability of CRC cells. a Long-term colony formation assay. On the left: representative images of colonies formed by DLD-1 cells after sequential treatments with 0.1 μM 5-aza-dC and 0.1–1 μM etoposide. On the right: the surviving fraction (SF) of DLD-1 cells after sequential treatments with 0.1 μM 5-aza-dC and 0.1–1 μM etoposide (n = 3). b Serial replating assay. On the left: schematic representation of the assay. The experiment ended on day 69 since the cells were treated with 0.1 μM 5-aza-dC. On the right: representative images of colonies formed by DLD-1 cells in the third plating after sequential treatments with 0.1 μM 5-aza-dC and 0.5 μM etoposide. D - days