Fig. 5

5-Aza-dC exerts long-lasting effects on CRC cells. a Cell viability of DLD-1 cells 5 (n = 15) and 13 (n = 4) days after exposure to 1 μM 5-aza-dC alone. Data are presented as means ± SD normalized to untreated control. b Representative bright-field microscopic images (bar = 100 μm) of DLD-1 cells 22 days after exposure to 1 μM 5-aza-dC alone. c Representative immunoblots of phospho-mTOR (Ser2481), mTOR, phospho-Akt (Ser473), Akt, phospho-p70S6K (Thr389), and p70S6K expression from DLD-1 cells 6 and 20 days after exposure to 0.1–1 μM 5-aza-dC alone (n ≥ 3). Actin served as a loading control. D - days