Fig. 3
Tanshinone IIA and ADM inhibited migration and invasion of A549 cells. Representative images of wound healing assay (a) and transwell assay (b) after 48 h treatment with 36 μM of tanshinone IIA (48 h IC50 value) and 1.5 μM of ADM (48 h IC50 value) alone or in combination. Bar graphs represent the average migration distance (c) and the number of stained cells (d) respectively, which were calculated from the three independent experiments with ten fields counted per experiment. Data are presented as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01, or ***P < 0.001 versus the vehicle control. (magnification, ×100. Scale bars, 100 μm)