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Fig. 5 | BMC Cancer

Fig. 5

From: Aberrant activation of Notch signaling in extrahepatic cholangiocarcinoma: clinicopathological features and therapeutic potential for cancer stem cell-like properties

Fig. 5

Anchorage-independent growth and mice tumorigenicity in the CC cell. lines pretreated with GSI IX ex vivo. a Colony formation in soft agar in RBE cells. b Normalized colony counts in RBE and TFK-1 cells pretreated with GSI IX. Pretreatment with both 20 and 40 μM of GSI IX significantly inhibited colony formations in the CC cell lines (P < 0.05). HuCCT1 cells did not form any colonies in soft agar after DMSO or GSI IX pretreatment (data not shown). c Representative photograph of engraftments of TFK-1 cells pretreated with GSI IX in NOD/SCID mice. Solid and dotted line arrow indicates GSI IX and DMSO, respectively. d Tumor growth curves of mice implantation in the CC cell lines with pretreatment of DMSO and GSI IX, respectively. Mice tumorigenicity in GSI IX pretreatment significantly delayed in all cell lines, compared to DMSO

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BMC Cancer

ISSN: 1471-2407

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