Fig. 2

miR-199a-5p inhibited cell migration and invasion ability in breast cancer cells (a, b) In vitro wound healing process was mimicked by scratching cells surface. The rate of migration was measured by quantifying the total distance that the cells moved from the edge of the scratch toward the center of the scratch (marked by imaginary lines). Cells were observed under x200 microscope at different time points (0, 3, 9 and 12 h. c, d Transwell invasion assay showed miR-199a-5p significant decreased the number of invasive cells (52 vs 33 cells per x400 field in control and miR-199a-5p mimic group, p = 0.0005). e Ectopic expression of miR-199a-5p significantly upregulated CDH1 expression and downregulated ZEB1, TWIST expression. All experiments were repeated in triplicates